Dose- and time-dependent increase of lysosomal enzymes in embryonic cartilage in vitro after ionizing radiation

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Scanning Microscopy; (USA)

OSTI ID:5798788

Cornelissen, M; de Ridder, L ^[1]

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1. State Univ. of Gent (Belgium)

Radiation doses of 20, 50 or 100 Gy caused the same time related decrease for RNA and proteoglycan (PG) synthesis in embryonic cartilage in vitro (4 days culture). In this paper, participation of lysosomes in this radiation response is investigated. Therefore, we employ a cytochemical method using beta-glycerophosphate as substrate for acid phosphatase (AP) detection. Increase of AP was found 2 days after irradiation and increased during the whole culture period. The increase was more pronounced with a higher radiation dose. Stimulation of AP activity explains the observed radiation response of RNA and PG synthesis.

• OSTI ID: 5798788
• Journal Information: Scanning Microscopy; (USA), Vol. 4:3; ISSN 0891-7035
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARTILAGE
BIOLOGICAL RADIATION EFFECTS
GLYCOPROTEINS
BIOSYNTHESIS
RNA
ACID PHOSPHATASE
CHICKENS
DOSE-RESPONSE RELATIONSHIPS
EMBRYOS
LYSOSOMES
RADIATION DOSES
TIME DEPENDENCE
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIRDS
BODY
CELL CONSTITUENTS
CONNECTIVE TISSUE
DOSES
ENZYMES
ESTERASES
FOWL
HYDROLASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHATASES
PROTEINS
RADIATION EFFECTS
SYNTHESIS
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture