Comparative study regarding the association of alpha 2U globulin with the nephrotoxic mechanism of certain petroleum-based air force fuels. Final report, 1 July 1990-31 August 1993

Technical Report · Tue Aug 31 04:00:00 EDT 1993

OSTI ID:5781856

Eurell, T E

Adult male rats have a strain, dose, and time-dependent renal proximal tubular degeneration induced by certain hydrocarbon compounds. We used rat strain variation (Fisher 344 and NCI Black Reiter) and different hydrocarbon compounds (JP-4, JP-8, decalin and trimethylpentane) to investigate the hydrocarbon-induced nephrotoxic response. Histochemical and morphometric evaluation of NCI-Black Reiter rats exposed to decalin and JP-8 indicated that this strain undergoes an intermediate form of the hydrocarbon-induced nephrotoxicity when compared to the albino Fisher 344 strain. The intermediate nephrotoxic response of the NCI-Black Reiter rat was characterized by approximately a two-fold increase in the number of acid phosphatase reactive lysosomes in renal tubular cells. The NBR rats did not demonstrate an increase in the size of the individual lysosomes, however, a characteristic lysosomal aggregation pattern occurred in renal tubular cells following hydrocarbon exposure. Light and electron microscopic immunohistochemistry revealed increased levels of A2U reactive sites in the renal tubular cell of F344 male rats exposed to decalin, trimethylpentane, JP-4 and JP-8. The nephrotoxic effect of decalin, trimethylpentane, JP-4 and JP-8 appeared to be equivalent as judged by renal tubular lysosomal alterations and increased A2U immunoreactive sites. Most lysosomal proteins in either control or treated animals were not reactive with specific antibodies against A2U. The relative number of A2U reactive sites per unit area did riot increase as lysosomes enlarged or became angular in response to hydrocarbon exposure. Alpha 2U globulin, Hyaline droplet nephropathy, Rats, Petroleum fuels, JP-4, JP-8, Cytoskeleton, Immunohistochemistry.

• Research Organization: Illinois Univ., Urbana, IL (United States). Coll. of Veterinary Medicine
• OSTI ID: 5781856
• Report Number(s): AD-A-269881/9/XAB; CNN: AFOSR-90-0303
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020600* -- Petroleum-- Health & Safety
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID PHOSPHATASE
ADULTS
AGE GROUPS
ALKANES
ANIMALS
ANTIBODIES
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BIOLOGY
BODY
BODY FLUIDS
CYCLOALKANES
DECALIN
DRUGS
ELECTRONS
ELEMENTARY PARTICLES
ENERGY SOURCES
ENZYMES
ESTERASES
FERMIONS
FOSSIL FUELS
FUELS
HYDROCARBONS
HYDROLASES
JET ENGINE FUELS
KIDNEYS
LEPTONS
LYSOSOMES
MALES
MAMMALS
MATERIALS
NATIONAL ORGANIZATIONS
ORGANIC COMPOUNDS
ORGANS
PETROLEUM
PH VALUE
PHOSPHATASES
PIGMENTS
PROTEINS
RATS
RODENTS
STRAINS
URINE
US DOD
US ORGANIZATIONS
VARIATIONS
VERTEBRATES
WASTES