Glomerular mesangium: kinetics using radiolabeled ferritin and the effects of aggregated IgG. [Rats]

Journal Article · Mon Oct 01 04:00:00 EDT 1984 · Clin. Immunol. Immunopathol.; (United States)

DOI:https://doi.org/10.1016/0090-1229(84)90294-0· OSTI ID:5776910

Borges, H F; Goldstein, C; Kim, M; Michael, A F

A quantitative method for studying glomerular mesangial kinetics using radiolabeled native ferritin (/sup 125/I-NF) is described. Groups of Sprague-Dawley rats were given injections of monomeric /sup 125/I-NF in a dose of 10 mg/100 g and sacrificed at 4, 16, and 36 hr. Radioactivity was measured in preparations of isolated glomeruli, spleen, and liver, and in blood. After initial uptake, a progressive decrease in the concentration of /sup 125/I-NF was observed in the mesangium and other organs. To examine the effect of one macromolecule on the mesangial kinetics of NF a separate group of animals was given aggregated human IgG (AHIgG) (40 mg/100 g) 4 hr prior to administration of /sup 125/I-NF. The administration of AHIgG did not alter the kinetics of /sup 125/I-NF in the mesangium, plasma, or spleen but the disappearance of /sup 125/I-NF from the liver was accelerated. Although the cellular mechanisms for uptake of NF and AHIgG are different, the presence of one macromolecule (AHIgG) within the mesangium did not affect the uptake and disappearance of another (NF). Thus, the glomerular mesangium has a relatively high capacity under normal circumstances.

• Research Organization: Univ. of Minnesota, Minneapolis
• OSTI ID: 5776910
• Journal Information: Clin. Immunol. Immunopathol.; (United States), Journal Name: Clin. Immunol. Immunopathol.; (United States) Vol. 33:1; ISSN CLIIA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
FERRITIN
GLANDS
GLOBULINS
GLOMERULI
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IRON COMPLEXES
ISOTOPES
KIDNEYS
LIVER
MAMMALS
MATERIALS
METALLOPROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RODENTS
SPLEEN
TRANSITION ELEMENT COMPLEXES
UPTAKE
VERTEBRATES