Mononuclear phagocytic system stimulation. Protective role from glomerular immune complex deposition. [Rats]

Journal Article · Thu Jan 01 04:00:00 EST 1981 · J. Lab. Clin. Med.; (United States)

OSTI ID:5546987

Raij, L; Sibley, R K; Keane, W F

Experimentally, glomerular deposition of circulating IC is increased when the MPS is saturated. Clinically, an association between glomerulonephritis and dysfunction of MPS-Fc receptor-mediated clearance of IC has recently been described in patients with certain forms of autoimmune diseases. Thus we hypothesized that stimulation of the MPS may be beneficial, by decreasing circulating IC and hence, reduce glomerular deposition of IC. To test this experimentally, we studied glomerular uptake and disappearance of AHIgG . /sup 125/I (macromolecular proteins biologically akin to IC) in normal control rats and in rats with ZY-stimulated MPS. Glomerular AHIgG . /sup 125/I was measured in preparations of isolated glomeruli and compared to simultaneous liver, spleen, lung, and blood greater than 7S AHIgG . /sup 125/I. The blood t 1/2 of greater 7S AHIgG . /sup 125/I in ZY rats was 40% shorter than that in control rats. Blood greater than 7S AHIgG . /sup 125/I in ZY rats was 63% lower than in control rats at 4 hr and 73% lower at 8 hr after intection. At all time intervals, glomerular AHIgG . /sup 125/I was reduced in ZY rats proportionately to the decreased blood levels. By immunofluorescence microscopy, the intensity of staining for human IgG correlated with the quantitative determination of AHIgG . /sup 125/I in preparations of isolated glomeruli in control and ZY rats. Serum complement depletion of ZY rats with CVF prior to AHIgG . /sup 125/I injection did not significantly alter the kinetics of AHIgG. /sup 125/I. This suggests that the increased MPS uptake of AHIgG . /sup 125/I in ZY rats was predominantly Fc-receptor-mediated. Thus ZY stimulation of the MPS increased the clearance of AHIgG . /sup 125/I and protected glomeruli from AHIgG . /sup 125/I deposition. Clinically, agents that would specifically stimulate the MPS may be useful in reducing IC-mediated glomerular injury.

• Research Organization: Department of Internal Medicine, University of Minnesota, Minneapolis
• OSTI ID: 5546987
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 98:4; ISSN JLCMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EXCRETION
GLANDS
GLOBULINS
GLOMERULI
IMMUNE REACTIONS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIVER
LUNGS
MAMMALS
MATERIALS
NEPHRITIS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTOSIS
PROTEINS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RENAL CLEARANCE
RESPIRATORY SYSTEM
RODENTS
SPLEEN
UROGENITAL SYSTEM DISEASES
VERTEBRATES