Clearance and renal transport of aggregated immunoglobulin in the mouse

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7188913

Getahun, B; Hartman, A; Hurtubise, P; Smith, R D

Heat aggregated human monoclonal IgG labeled with I/sup 125/ was used to examine serum clearance, uptake by various organs and appearance in the urine in Balb/C mice. Following injection of 5 mg of the low molecular weight fraction (1.8 x 10/sup 5/), the IgG was taken up by the liver and spleen by six hours and then gradually excreted in increasing concentrations in the urine. The concentration in blood, liver, spleen and kidney dropped progressively after 6 hours. Injection of the high molecular weight fraction (1.3 x 10/sup 6/) resulted in rapid disappearance from the serum with high concentrations in liver, kidney, and spleen by 2 hours. Serum levels then increased reaching a peak in 6 hours. Serum levels then increased reaching a peak in 6 hours. Using immunoelectron microscopy, IgG was localized to the glomerular mesangium, glomerular capillaries and tubular epithelial cells as early as one hour after injection. The label also detected IgG in periglomerular and peritubular spaces. After 12 hours, residual IgG was observed principally in mesangial and peritubular spaces. Injection of the low molecular weight fraction did not result in accumulation of IgG in glomeruli. These studies suggest that there are two major renal routes of clearance of aggregated IgG. The low MW fraction is cleared through the tubules presumably following transport across the glomerular capillary wall. The heavy MW component is cleared principally through the mesangium and periglomerular and peritubular channels, presumably lymphatics.

• Research Organization: Univ. of Cincinnati, OH
• OSTI ID: 7188913
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
EXCRETION
GLANDS
GLOBULINS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIVER
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
MICE
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RENAL CLEARANCE
RODENTS
SPLEEN
TRACER TECHNIQUES
UPTAKE
VERTEBRATES