Rapid rejoining of DNA strand breaks in resting human lymphocytes after irradiation by low doses of /sup 60/Co. gamma. rays or 14. 6-MeV neutrons

Journal Article · Wed Jun 01 04:00:00 EDT 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575907· OSTI ID:5775200

McWilliams, R S ^[1]; Cross, W G; Kaplan, J G; Birnboim, H C

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1. Univ. of Ottawa, Ontario

The production and repair of DNA strand breaks was studied in human lymphocytes by means of a sensitive fluorometric technique. Lymphocytes were isolated by conventional methods and air-equilibrated suspensions were irradiated by low doses (less than or equal to 2 Gy) of either /sup 60/Co ..gamma.. rays or 14.6-MeV neutrons at 0/sup 0/C. The apparent yield of initial strand breaks induced by neutrons was only 36% of that induced by ..gamma.. rays, in agreement with the observations of other workers. Resting lymphocytes were found to be proficient in their ability to rejoin ..gamma..-induced strand breaks at 37/sup 0/C; rejoining followed biphasic kinetics, with 70% of the breaks disappearing with a half-life of about 3 min. Although the initial number of breaks induced by neutron irradiation was low, after 20 min of incubation the residual number of breaks was very similar for the two forms of radiation.

• OSTI ID: 5775200
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 94:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BARYONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DATA
DNA
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ENERGY RANGE
EXPERIMENTAL DATA
FAST NEUTRONS
FERMIONS
GAMMA RADIATION
HADRONS
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MEV RANGE
MEV RANGE 10-100
MINUTES LIVING RADIOISOTOPES
NEUTRONS
NUCLEI
NUCLEIC ACIDS
NUCLEONS
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RECOVERY
REPAIR
SOMATIC CELLS
STRAND BREAKS
VERTEBRATES
YEARS LIVING RADIOISOTOPES