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Active transport of lead-210 by everted segments of rat duodenum

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:5774834
Everted gut sacs prepared from rat duodenum can transfer /sup 210/Pb from mucosal to serosal surfaces against concentration gradients in vitro. The mechanism requires oxygen and is significantly inhibited by sodium azide, sodium iodoacetate, 2,4-dinitrophenol, sodium cyanide and sodium fluoride. Lack of oxygen or the presence of metabolic inhibitors produced greater relative inhibition of net serosal transfer but greater absolute decrements of net mucosal uptake. Treatment of rats prior to sac preparation with either intravenous FeCl/sub 2/ or endotoxin, or the addition of FeCl/sub 2/ to the mucosal medium, selectively inhibited net serosal transfer. Net mucosal uptake and net serosal transfer were significantly negatively correlated with animal weight. Jejunal or ileal sacs did not actively transport /sup 210/Pb.
Research Organization:
Univ. of Alabama, Birmingham
OSTI ID:
5774834
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 247:2; ISSN AJPHA
Country of Publication:
United States
Language:
English