IN VITRO ABSORPTION OF RADIOIRON BY EVERTED POUCHES OF RAT INTESTINE

Journal Article · Fri Aug 01 04:00:00 EDT 1958 · American Journal of Physiology (U.S.)

OSTI ID:4308675

Brown, Jr, E B; Justus, B W

Everted pouches of rat intestine prepared by the technique of Wilson and associates were used to measure absorption of radiation. Iron was taken up equally well in vitro by all segments of the rat's small intestine; but when the iron was given orally in vivo, a distinct gradient, highest in duodenum and progressively smaller in the distal segments was demonstrated. There was little transfer into the inner serosal pouch. Transfer of iron in this preparation was by a process of passive transport. It was not appreciably affected by changes in pH, various metabolic inhibitors, buffer systems, or added substrates. Ferrous iron was sigificantly better taken up by the intestinal tissue, but transfer into ihe inner pouch fluid was greater with ferric iron. (auth)

• Research Organization: Washington University School of Medicine, St. Louis
• NSA Number: NSA-12-016168
• OSTI ID: 4308675
• Journal Information: American Journal of Physiology (U.S.), Journal Name: American Journal of Physiology (U.S.) Vol. Vol: 194; ISSN AJPHA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACIDITY
BIOLOGY AND MEDICINE
CHEMICALS
DIFFUSION
DISTRIBUTION
DUODENUM
INTESTINE
IONS
IRON ISOTOPES
LIQUIDS
MEASURED VALUES
MEMBRANES
METABOLISM
RATS
SURGERY
VARIATIONS