Effects of adenosine infusion into renal interstitium on renal hemodynamics

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:5765401

Pawlowska, D; Granger, J P; Knox, F G

This study was designed to investigate the hemodynamic effects of exogenous adenosine in the interstitium of the rat kidney. Adenosine or its analogues were infused into the renal interstitium by means of chronically implanted capsules. In fusion of adenosine decreased glomerular filtration rate (GFR) from 0.81 +/- 0.06 to 0.37 +/- 0.06 ml/min while having no effect on renal blood flow (RBF). The metabolically stable analogue, 2-chloradenosine (2-ClAdo), decreased GFR from 0.73 +/- 0.07 to 021 +/- 0.06 ml/min. Interstitial infusion of theophylline, an adenosine receptor antagonist, completely abolished the effects of adenosine and 2-ClAdo on GFR. The distribution of adenosine, when infused into the renal interstitium, was determined using radiolabeled 5'-(N-ethyl)-carboxamidoadenosine (NECA), a metabolically stable adenosine agonist. After continuous infusion, (/sup 3/H)NECA was distributed throughout the kidney. The effects of NECA to reduce GFR were similar to those of adenosine and 2-ClAdo. They conclude that increased levels of adenosine in the renal interstitium markedly decrease GFR without affecting RBF in steady-state conditions. The marked effects of adenosine agonists during their infusion into the renal interstitium and the complete blockade of these effects by theophylline suggest an extracellular action of adenosine.

• Research Organization: Mayo Medical School, Rochester, MN
• OSTI ID: 5765401
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:4; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ANIMALS
BODY
CARBOHYDRATES
CLEARANCE
DISTRIBUTION
EXCRETION
FILTRATION
GLOMERULI
INFUSION
INTAKE
INULIN
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RATS
RENAL CLEARANCE
RIBOSIDES
RODENTS
SACCHARIDES
SEPARATION PROCESSES
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
ULTRAFILTRATION
VERTEBRATES