Re-evaluation of 1,2-dimethylhydrazine in the mouse bone marrow micronucleus assay: observation of a positive response

Journal Article · Wed Dec 31 23:00:00 EST 1986 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860090208· OSTI ID:5764549

Ashby, J; Mirkova, E

1,2-Dimethylhydrazine showed dose-related clastogenic activity in the mouse bone marrow micronucleus assay following intubation of an aqueous solution of the dihydrocholoride salt (10 and 50 mg/kg, 24 h sampling). These data counter earlier reports of the inactivity of this material in a rat and a mouse micronucleus assay, following its intraperitoneal injection. In the present study, 2000 polychromatic erythrocytes were assessed per animal. However, had only 300 cells been assessed for micronucleated polychromatic erythrocytes, it is shown that a negative result could have been recorded. This may explain the earlier and negative results.

• Research Organization: Imperial Chemical Industries PLC, Chesire, England
• OSTI ID: 5764549
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 9:2; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
AQUEOUS SOLUTIONS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CELL CONSTITUENTS
CELL NUCLEI
DISPERSIONS
ERYTHROCYTES
HEMATOPOIETIC SYSTEM
HYDRAZINE
MAMMALS
MATERIALS
METABOLIC ACTIVATION
MICE
MIXTURES
NITROGEN COMPOUNDS
ORGANS
RODENTS
SOLUTIONS
TISSUES
VERTEBRATES