Assessment of 1,2-dimethylhydrazine in bone marrow micronucleus assay: Variations in protocol and response
- Univ. of Guelph, Ontario (Canada)
The effect of variations in experimental protocol on the assessment of the genotoxicity of 1,2-dimethylhydrazine (DMH) in the bone marrow micronucleus assay was determined. The incidence of micronuclei (MN) in the bone marrow of CBA mice treated with DMH (either intraperitoneally (i.p.) or orally) was found to be significantly greater than that observed in C57B1/6J mice using the same dose and dosing regimen. With i.p. injection, DMH, at doses of 20 and 50 mg/kg, was found to be positive in the bone marrow MN test in CBA mice only. In C57B1/6J mice, DMH (i.p.) was found to be positive at only the 50 mg/kg dose. With oral administration, DMH was positive in the MN test only at the 50 mg/kg dose and only in CBA mice. Cyclophosphamide (CY) was found to induce a dose-dependent increase in the percentage of MN observed in the bone marrow of C57B1/6J mice. DMH tested positive in the colon nuclear aberration (NA) assay in both strains of mice using both i.p. and oral routes of administration, although C57B1/6J mice were found to be more sensitive than CBA mice. No significant difference was observed regarding the percentage of NAs observed in the colon between mice injected i.p. or orally gavaged.
- OSTI ID:
- 5929925
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Vol. 17:3; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE MARROW CELLS
DOSE-RESPONSE RELATIONSHIPS
HYDRAZINE
GENETIC EFFECTS
ENDOXAN
INTRAPERITONEAL INJECTION
MICE
ORAL ADMINISTRATION
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CONNECTIVE TISSUE CELLS
DRUGS
IMMUNOSUPPRESSIVE DRUGS
INJECTION
INTAKE
MAMMALS
NITROGEN COMPOUNDS
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology