Assessment of 1,2-dimethylhydrazine in bone marrow micronucleus assay: Variations in protocol and response

Journal Article · Tue Jan 01 04:00:00 UTC 1991 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850170304· OSTI ID:5929925

Goldberg, M T; Schop, R N ^[1]

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1. Univ. of Guelph, Ontario (Canada)

The effect of variations in experimental protocol on the assessment of the genotoxicity of 1,2-dimethylhydrazine (DMH) in the bone marrow micronucleus assay was determined. The incidence of micronuclei (MN) in the bone marrow of CBA mice treated with DMH (either intraperitoneally (i.p.) or orally) was found to be significantly greater than that observed in C57B1/6J mice using the same dose and dosing regimen. With i.p. injection, DMH, at doses of 20 and 50 mg/kg, was found to be positive in the bone marrow MN test in CBA mice only. In C57B1/6J mice, DMH (i.p.) was found to be positive at only the 50 mg/kg dose. With oral administration, DMH was positive in the MN test only at the 50 mg/kg dose and only in CBA mice. Cyclophosphamide (CY) was found to induce a dose-dependent increase in the percentage of MN observed in the bone marrow of C57B1/6J mice. DMH tested positive in the colon nuclear aberration (NA) assay in both strains of mice using both i.p. and oral routes of administration, although C57B1/6J mice were found to be more sensitive than CBA mice. No significant difference was observed regarding the percentage of NAs observed in the colon between mice injected i.p. or orally gavaged.

• OSTI ID: 5929925
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 17:3; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENDOXAN
GENETIC EFFECTS
HYDRAZINE
IMMUNOSUPPRESSIVE DRUGS
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
MAMMALS
MICE
NITROGEN COMPOUNDS
ORAL ADMINISTRATION
RODENTS
SOMATIC CELLS
VERTEBRATES