Circulating factors that modify lung cell DNA synthesis following exposure to inhaled oxidants. I. Effect of serum and lavage on lung fibroblasts following exposure of adult rats to 1 ppm ozone

Journal Article · Sun Jan 01 00:00:00 EST 1989 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398909531294· OSTI ID:5764359

Tanswell, A K; Fraher, L J; Grose, E C

Adult rats were exposed to 1 ppm (1.96 mg/m/sup 3/) ozone or air for 2 wk. Animals were sacrificed at 3, 5, 7, or 14 d after the onset of exposure, and samples of plasma and lung lavage were obtained. Heat-inactivated plasma, from animals exposed to ozone for 7 or 14 d, significantly increased DNA synthesis by lung fibroblasts compared with plasma from air-exposed animals. Fractionation of plasma and lavage samples indicated that the factor responsible had an isoelectric point of 6.45-6.75 and a molecular weight of 32 +/- 2 kDa. This factor has a dose-dependent effect on lung fibroblast DNA synthesis in culture, but no significant effect on cultured pneumocyte DNA synthesis. The factor is detectable within 72 h of exposure, and may hold some promise as a marker of early oxidant lung injury.

• Research Organization: Univ. of Western Ontario, London (Canada)
• OSTI ID: 5764359
• Journal Information: J. Toxicol. Environ. Health; (United States), Vol. 27:2
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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LUNGS
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OZONE
TOXICITY
BIOASSAY
BLOOD PLASMA
CELL CULTURES
CHROMATOGRAPHY
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MAMMALS
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560300* - Chemicals Metabolism & Toxicology