Dose fractionation effects in plateau-phase cultures of C3H 10T1/2 cells and their transformed counterparts

Journal Article · Fri Feb 01 04:00:00 EST 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576402· OSTI ID:5763850

Zeman, E M; Bedford, J S

A comparison of ..gamma..-ray dose fractionation effects was made using plateau-phase cultures of C3H 10T1/2 cells and their transformed counterparts in an attempt to simulate basically similar populations of cells that differ primarily in their turnover rates. The status of cell populations with respect to their turnover rates may be an important factor influencing dose fractionation effects in early- and late-responding tissues. In this cell culture system, the rate of cell turnover was approximately three times higher for the plateau-phase transformed cultures. While the single acute dose survival curves for log-phase cells were indistinguishable, there were significant differences between the survival curves for plateau-phase cultures of the two cell types. Both cell lines had a similar capacity for repair of sublethal damage, but untransformed cells had a much greater capacity to repair potentially lethal damage in plateau phase. Multifraction survival curves were determined for both cell lines for doses per fraction ranging from 9.0 to 0.8 Gy, and from these isoeffect curves of log total dose versus dose per fraction were derived. The isoeffect curve for the slowly cycling, untransformed cells was found to be appreciably steeper than that for the more rapidly cycling transformed cells, a finding consistent with previously reported differences in dose fractionation isoeffect curves for early- and late-responding tissues in vivo.

• Research Organization: Colorado State Univ., Fort Collins
• OSTI ID: 5763850
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 101:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
CELL CYCLE
CELL KILLING
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DATA
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
FIBROBLASTS
FRACTIONATED IRRADIATION
IN VITRO
INFORMATION
IRRADIATION
KINETICS
LABELLED COMPOUNDS
NUMERICAL DATA
ONCOGENIC TRANSFORMATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
SOMATIC CELLS
TRITIUM COMPOUNDS