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Neoplastic transformation of plateau-phase mouse 10T1/2 cells following single and fractionated doses of X rays

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576712· OSTI ID:5406225
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The induction of cell transformation and cell killing in plateau-phase 10T1/2 mouse cells by single and fractionated doses of X rays was investigated. The single dose-transformation curve was composed of a first phase (doubling dose = 0.2 Gy), a second phase (doubling dose = 1 Gy), and a third phase (no increase of transformation at doses more than 6 Gy). Two-dose fractionation experiments revealed that transformation damage was reduced when total doses of 0.93, 1.86, and 3.72 Gy were given with two equal fractions separated by intervals of 3 to 15 hr, as compared with the equivalent single doses. This not only represents repair of subtransformation damage but also is consistent with previous findings of repair of potential transformation damage. The fractionated dose-transformation curve obtained 3 hr after the first (conditioning) dose indicated that damaged cells had recovered, at least in part, from transformation damage, as shown by the reappearance of the first phase of transformation induction. From the present results with single and fractionated doses, a resolution between repair of sub- and potential transformation damage did not seem possible. Plateau-phase cells were found to be useful for dose fractionation studies.
Research Organization:
National Institute of Radiological Sciences, Chiba-si, Japan
OSTI ID:
5406225
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 102:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ACUTE IRRADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CYCLE
CELL KILLING
CONNECTIVE TISSUE CELLS
DATA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROBLASTS
FRACTIONATED IRRADIATION
INFORMATION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
MICE
NUMERICAL DATA
ONCOGENIC TRANSFORMATIONS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
VERTEBRATES
X RADIATION