Inducible responses to DNA damage in the mouse embryo fibroblasts cell line C3H/10T1/2 and its transformed counterpart C3H/MCA
Early passage mouse embryo fibroblasts cells (C3H/10T1/2) were treated with ultraviolet (UV) radiation of 12-O-tetradecanoylphorbol-13-acetate (TPA) in order to determine whether such treatment induced DNA repair processes, measured as increased survival and mutagenesis of Herpes simplex (HSV-1). No enhanced host cell reactivation of UV-irradiated virus was observed following treatment of cells with UV-irradiation or TPA. Replication of undamaged virus in untreated C3H cells resulted in an increase over the background mutation frequency. When the cells were UV-irradiated and infected with unirradiated virus, a decrease in mutagenesis was observed. Decreased untargeted mutagenesis was shown to be dose- and time-dependent, reaching a minimum at a fluence of 5-7 Jm/sup /minus/2/ for 24 hours between irradiation and infection of cells. There was no change in mutagenesis of UV-irradiated virus grown in UV-irradiated cells compared to untreated cells. The repair capacity of methylcholanthrene-transformed C3H cells (MCA cells) was compared with untransformed C3H cells. The cell lines demonstrated similar cell survival curves following UV-irradiation but differed markedly in their ability to repair damaged HSV-1.
- Research Organization:
- Pennsylvania State Univ., University Park (USA)
- OSTI ID:
- 6148510
- Country of Publication:
- United States
- Language:
- English
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Cells
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560130 -- Radiation Effects on Microorganisms
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARCINOGENS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EMBRYOS
ESTERS
FIBROBLASTS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HERPES SIMPLEX
INFECTIOUS DISEASES
MAMMALS
MICE
MICROORGANISMS
MUTATION FREQUENCY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHORBOL ESTERS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
RODENTS
SKIN DISEASES
SOMATIC CELLS
SURVIVAL CURVES
TIME DEPENDENCE
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
VIRUSES