Ceruloplasmin inhibits carbonyl formation in endogenous proteins in phorbol myristate acetate (PMA)-stimulated neutrophils

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5751707

Krsek-Staples, J; Webster, R O ^[1]

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1. St. Louis Univ., MO (United States)

The respiratory burst of stimulated neutrophils can cause oxidative modifications of endogenous neutrophil proteins as measured by increased carbonyl formation. Ceruloplasmin is an acute phase protein and may act as an antioxidant during inflammation. Therefore, the role of ceruloplasmin in preventing oxidative damage of endogenous neutrophil proteins was investigated. Protein carbonyl content was determined spectrophotometrically using 2,4-dinitrophenylhydrazine. Ceruloplasmin, at a concentration present during inflammation significantly inhibited carbonyl formation in endogenous proteins of PMA-stimulated neutrophils. In order to determine if oxidative damage was occurring to the ceruloplasmin upon incubation with stimulated neutrophils, carbonyl formation in the ceruloplasmin in the presence and absence of stimulated neutrophils. This data suggests that ceruloplasmin may play a role in regulating oxidative damage to proteins and that ceruloplasmin itself may act as a target for these modifications.

• OSTI ID: 5751707
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBONYLS
CARCINOGENS
CERULOPLASMIN
COMPLEXES
COPPER COMPLEXES
ESTERS
GLOBULINS
GLOBULINS-ALPHA
LEUKOCYTES
MATERIALS
METALLOPROTEINS
NEUTROPHILS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PROTEINS
SPECTROPHOTOMETRY
SYNTHESIS
TRANSITION ELEMENT COMPLEXES