Activation of the NADPH oxidase in a cell-free system from human neutrophils stimulated by phorbol myristate acetate

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90447-Y· OSTI ID:6967648

Umeki, Shigenobu ^[1]

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1. Kawasaki Medical School, Okayama (Japan)

Kinetics of activation of the NADPH oxidase in a fully soluble cell-free system from phorbol myristate acetate (PMA)-stimulated human neutrophils were investigated. In a cell-free system in which Mg{sup 2+} and sodium dodecyl sulfate, an anionic detergent required for the activation of NADPH oxidase are contained, cytosol prepared from PMA-stimulated neutrophils failed to activate PMA-stimulated neutrophil oxidase. However, cytosol prepared from resting (control) neutrophils was capable of activating PMA-stimulated neutrophil oxidase in a cell-free system in which its Km for NADPH was almost similar to that of control neutrophil oxidase. Cytosol from PMA-stimulated neutrophils could not activate control neutrophil oxidase, although it did not contain any inhibitors of NADPH oxidase activation. These results suggest that, in PMA-stimulated neutrophils, cytosolic activation factors may be consumed or exhausted, and that the affinity for NADPH of PMA-stimulated neutrophil oxidase may be the same as that of control neutrophil oxidase.

• OSTI ID: 6967648
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 46:16; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

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