Ceruloplasmin inhibits endothelial cell injury and hydroxyl radical production by phorbol myristate acetate (PMA)-stimulated neutrophils

Conference · Mon Feb 26 04:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6054102

Krsek-Staples, J; Webster, R O ^[1]

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1. St. Louis Univ., MO (United States)

Stimulated neutrophils produce oxygen radicals which may cause injury to endothelial cells during inflammatory diseases. Ceruloplasmin is an acute phase protein in which serum levels can increase 2-3 fold during inflammation. Ceruloplasmin may act as an extracellular antioxidant by oxidizing iron and mediating iron-dependent oxygen radical production. The role of ceruloplasmin in reducing injury to endothelial cells and hydroxyl radical production by PMA-stimulated neutrophils was investigated. Ceruloplasmin was purified from human plasma using standard column chromatography methods. Injury to bovine pulmonary using standard column chromatography methods. Injury to bovine pulmonary artery endothelial (BPAE) cells was measured using a {sup 51}chromium release assay. {sub 51}Chromium release from endothelial cells incubated with PMA-stimulated neutrophils was significantly inhibited by 500 {mu}g/ml ceruloplasmin. Hydroxyl radical production by neutrophils was measured using a spectrophotometric deoxyribose degradation assay. Ceruloplasmin at 500 {mu}g/ml significantly stimulated neutrophils. These results indicate that ceruloplasmin may play a role in regulating stimulated neutrophil-mediated endothelial cell injury possibly by preventing the formation of hydroxyl radicals.

• OSTI ID: 6054102
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
ANTIOXIDANTS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLASMA
BODY
BODY FLUIDS
CARCINOGENS
CERULOPLASMIN
CHEMICAL ANALYSIS
CHROMIUM 51
CHROMIUM ISOTOPES
COMPLEXES
COPPER COMPLEXES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ESTERS
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-ALPHA
HYDROXYL RADICALS
INFLAMMATION
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPES
KINETICS
LEUKOCYTES
MAMMALS
MAN
MATERIALS
METALLOPROTEINS
NEUTROPHILS
NUCLEI
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHORBOL ESTERS
PRIMATES
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADICALS
RADIO-RELEASE ANALYSIS
RADIOISOTOPES
REACTION KINETICS
SYMPTOMS
TISSUES
TOXICITY
TRANSITION ELEMENT COMPLEXES
VERTEBRATES