Mutagenesis assays of human amniotic fluid
Journal Article
·
· Environ. Mutagen.; (United States)
Extracts of amniocentesis samples from 144 women were tested for the presence of mutagenic substances using tester strain TA1538 in the Ames Salmonella/mammalian-microsome mutagenicity test. Because the volume of amniotic fluid in these samples was limited (generally less than 10 ml), the authors investigated modifications of this mutagenesis assay that could increase its ability to detect effects from small quantities of test material. Using mutagenicity in samples of urine from smokers as a model, it appeared that improved ability to detect small amounts of mutagen could be obtained by reducing volumes of media and reagents while keeping the amount of test sample constant. Tests of amniotic fluid extracts by this modified procedure showed small increases in revertants, about 50% above dimethylsulfoxide solvent control values. The increases suggest the presence of small amounts of mutagenic material in many of the amniotic fluid samples. At the doses employed, mutagenic activity in these samples was not associated with maternal smoking.
- Research Organization:
- National Institute of Environmental Health Sciences, Research Triangle Park, NC
- OSTI ID:
- 5748281
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 7:2; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
AMNIOTIC FLUID
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
COLLOIDS
DISPERSIONS
FEMALES
GENETIC EFFECTS
MAMMALS
MAN
MATERIALS
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
MUTANTS
PREGNANCY
PRIMATES
RESIDUES
REVERTANTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SMOKES
SOLS
TOBACCO SMOKES
URINE
VERTEBRATES
WASTES
WOMEN
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
AMNIOTIC FLUID
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
COLLOIDS
DISPERSIONS
FEMALES
GENETIC EFFECTS
MAMMALS
MAN
MATERIALS
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
MUTANTS
PREGNANCY
PRIMATES
RESIDUES
REVERTANTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SMOKES
SOLS
TOBACCO SMOKES
URINE
VERTEBRATES
WASTES
WOMEN