In vitro mutagenicity assays of chemical carcinogens and related compounds with Salmonella typhimurium

Journal Article · Sun Apr 01 04:00:00 UTC 1979 · J. Natl. Cancer Inst.; (United States)

OSTI ID:5998329

Simmon, V F

The mutagenic activity of 101 chemicals was studied with the use of the Salmonella typhimurium-microsome system described by Ames. The tester strains were TA1535, TA1536, TA1537, TA1538, TA98, and TA100. Assays were conducted in the presence and absence of a metabolic activation system prepared from the livers of randomly bred Sprague-Dawley rats that had been pretreated with Aroclor 1254. The test chemicals were incorporated into the agar with bacteria and the metabolic activation system. Mutagens were defined as chemicals that induced a reproducible dose-related increase in the number of histidine-independent revertants. With the use of these procedures, 65% of the organic carcinogens and 25% of the noncarcinogens were found to be mutagenic.

• Research Organization: Stanford Research Inst., Menlo Park, CA
• OSTI ID: 5998329
• Journal Information: J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 62:4; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300 -- Cytology
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOASSAY
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CONSTITUENTS
DOSE-RESPONSE RELATIONSHIPS
GENETIC EFFECTS
IN VITRO
MICROORGANISMS
MICROSOMES
MUTAGEN SCREENING
MUTAGENESIS
MUTAGENS
ORGANOIDS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING