Diffusion of intracerebrally injected (1-/sup 14/C)arachidonic acid and (2-/sup 3/H)glycerol in the mouse brain. Effects of ischemia and electroconvulsive shock

Journal Article · Wed Dec 01 04:00:00 EST 1982 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00965088· OSTI ID:5744999

Pediconi, M F; Rodriguez de Turco, E B; Bazan, N G

(2-/sup 3/H)Glycerol and (1-/sup 14/C)arachidonic acid were injected into the region of the frontal horn of the left ventricle of mice and were distributed rapidly throughout the brain. After 10 sec, most of the radioactive fatty acid was found in the hemisphere near the injection site; after 10 min, it was recovered in similar proportions in the cerebellum and brain stem. (2-/sup 3/H)Glycerol showed a heterogeneous distribution, with most of the label remaining in the left hemisphere even after 10 min. On a fresh weight basis, cerebrum, cerebellum, and brain stem were found to contain similar amounts of labeled glycerol. However, the amount of (1-/sup 14/C)arachidonate in cerebrum was only 50% of that recovered from cerebellum or brain stem. Brain ischemia or a single electroconvulsive shock reduced the spread of the label, producing an accumulation of radioactivity in the injected hemisphere, except for an increase in (2-/sup 3/H)glycerol in the brain stem during ischemia. Despite the significant decrease in available precursor in the cerebellum and brain stem after electroshock, the amount of label incorporated into lipids was not altered in these areas and only slightly diminished in the cerebrum.

• Research Organization: Instituto de Investigaciones Bioquimicas, Universidad Nacional del Sur-Consejo Nacional de Investigaciones Cientificas y Technicas, Bahia Blanca, Argentina
• OSTI ID: 5744999
• Journal Information: Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 7:12; ISSN NERED
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMALS
ARACHIDONIC ACID
BODY
BRAIN
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CEREBELLUM
CEREBRUM
DIFFUSION
DISEASES
DISTRIBUTION
ELECTRIC SHOCK
GLYCEROL
HYDROXY COMPOUNDS
ISCHEMIA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MICE
MONOCARBOXYLIC ACIDS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASCULAR DISEASES
VERTEBRATES