Reduced labeling of brain phosphatidylinositol, triacylglycerols, and diacylglycerols by (1-/sup 14/C)arachidonic acid after electroconvulsive shock: potentiation of the effect by adrenergic drugs and comparison with palmitic acid labeling

Journal Article · Sat Feb 01 04:00:00 EST 1986 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00967970· OSTI ID:5429612

Pediconi, M F; Rodriguez de Turco, E B; Bazan, N G

The effect of electroconvulsive shock on the labeling of phospholipids and neutral lipids in mice brains was examined after intracerebral injection of (1-/sup 14/C) arachidonic acid or (1-/sup 14/C)palmitic acid. Electroconvulsive shock reduced greatly the removal of radiolabeled arachidonic acid from the free fatty acid pool. At the same time, the incorporation of arachidonic acid was partially inhibited in triacylglycerol, diacylglycerol, and phosphatidylinositol, whereas the incorporation of (1-/sup 14/C)palmitic acid was not affected. Pretreatment with desipramine and pargyline potentiated the lipid effect of electroconvulsive shock in neutral glycerides. These electroconvulsive shock-induced changes reflect alterations in the metabolism of intracerebrally injected arachidonic acid, but not of similarly injected palmitic acid. From the available data whether decreased ATP, enzyme inhibition or other factors are involved cannot be ascertained. Moreover, the electroconvulsive shock-enhanced endogenous free arachidonic acid may possibly dilute the injected radiolabeled fatty acid, thus decreasing its availability for arachidonoyl-coenzyme A synthesis. Hence, a partial inhibition of the activation-acylation of these fatty acids, primarily arachidonic acid, also may be involved in the seizure-induced accumulation of free fatty acids in the brain.

• Research Organization: Universidad Nacional del Sur-Consejo Nacional de Investigaciones Cientificas y Tecnicas, Bahia Blanca, Argentina
• OSTI ID: 5429612
• Journal Information: Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 2; ISSN NERED
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BIOLOGICAL SHOCK
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
COMPARATIVE EVALUATIONS
ESTERS
HEXADECANOIC ACID
INOSITOLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LIPIDS
MAMMALS
METABOLISM
MICE
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
RODENTS
SACCHARIDES
TRACER TECHNIQUES
TRIGLYCERIDES
VERTEBRATES