Calcium-dependent phospholipid catabolism and arachidonic acid mobilization in cerebral minces

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Molecular and Chemical Neuropathology; (USA)

DOI:https://doi.org/10.1007/BF03159943· OSTI ID:5544195

Damron, D S; Dorman, R V ^[1]

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1. Kent State Univ., OH (USA)

Cerebral minces were used to investigate the role of calcium influx on trauma-induced alterations of brain lipid metabolism. Cerebral phospholipids, nonpolar lipids, and free fatty acids were radiolabeled in vivo with ({sup 3}H)arachidonic acid. Tissue incubation stimulated the time-dependent catabolism of choline and inositol glycerophospholipids, and resulted in the accumulation of ({sup 3}H)free fatty acids. These effects were attenuated in Ca{sup 2}{sup +}-free incubations, and when EGTA or verapamil were present. The inhibition of calcium influx also reduced the labeling of diglycerides, whereas ethanolamine and serine glycerophospholipids were not affected by incubation or treatments. Replacing Ca{sup 2}{sup +} with other cations also attenuated the incubation-dependent alterations in lipid metabolism. However, only cadmium was able to compete with calcium and reduce the accumulation of ({sup 3}H)free fatty acids. It appeared that about half of the observed phospholipid catabolism was dependent on Ca{sup 2}{sup +} influx and that at least 80% of the ({sup 3}H)free fatty acid accumulation required calcium.

• OSTI ID: 5544195
• Journal Information: Molecular and Chemical Neuropathology; (USA), Journal Name: Molecular and Chemical Neuropathology; (USA) Vol. 12:3; ISSN 1044-7393; ISSN MCHNE
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALKALINE EARTH METALS
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL ACCUMULATION
BIOLOGICAL FUNCTIONS
BODY
BRAIN
CALCIUM
CARBOXYLIC ACIDS
CATABOLISM
CENTRAL NERVOUS SYSTEM
CHELATING AGENTS
EGTA
ELEMENTS
ESTERS
GLYCOLS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INJURIES
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LIPIDS
MAMMALS
METABOLISM
METALS
MONOCARBOXYLIC ACIDS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RATS
RODENTS
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES