Activation of phosphatidic acid metabolism of human erythrocyte membranes by perfringolysin O

Journal Article · Thu May 29 04:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)91170-8· OSTI ID:5003087

Saito, M; Ando, S; Mitsui, K; Homma, Y; Takenawa, T

The effect of perfringolysin O on the lipid metabolism of human erythrocyte membranes was investigated. Erythrocytes were prelabeled with (/sup 3/H)arachidonic acid and (/sup 32/P)inorganic phosphate. In the presence of calcium ion (5.5 mM), the effect of perfringolysin O on lipid metabolism was very similar to that of an calcium-ionophore A23187. In the absence of calcium ion, the accumulation of phosphatidic acid and its following decreasing trend were observed during the reaction with the toxin. Such changes were not caused by filipin. These results suggest that perfringolysin O causes the activation of a diglyceride-phosphatidic acid cycle, which might be involved in the calcium transport.

• Research Organization: Tokyo Metropolitan Institute of Gerontology, Japan
• OSTI ID: 5003087
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 137:1; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METAL COMPOUNDS
ANTIGENS
ARACHIDONIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM COMPOUNDS
CARBOXYLIC ACIDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMICAL ACTIVATION
DATA
DAYS LIVING RADIOISOTOPES
ERYTHROCYTES
EXPERIMENTAL DATA
INFORMATION
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
MATERIALS
MEMBRANE TRANSPORT
MEMBRANES
METABOLISM
MONOCARBOXYLIC ACIDS
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RESPONSE MODIFYING FACTORS
TOXIC MATERIALS
TOXICITY
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS