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Increased sensitivity of lymphocytes from people over 65 to cell cycle arrest and chromosomal damage

Journal Article · · Science (Washington, D.C.); (United States)
; ; ; ; ;

Flow cytometry revealed that, in the presence of tritiated thymidine, a greater percentage of phytohemagglutinin-stimulated lymphocytes from old human donors were arrested in the G2 or M phase than were cells from young donors. Furthermore, lymphocytes from old donors showed significantly more chromosomal damage than did lymphocytes from young donors. Lymphocyte cultures from old or young donors not exposed to tritiated thymidine had the same percentage of cycling lymphocytes in G2 or M, although the number of lymphocytes stimulated by phytohemagglutinin to enter the cell cycle was significantly lower in cultures from old donors. Thus, the impaired incorporation of tritiated thymidine by phytohemagglutinin-exposed lymphocytes from old humans reflects both an impaired proliferative response to phytohemagglutinin and an increased sensitivity to the radiobiological effects of tritiated thymidine.

Research Organization:
Sloan-Kettering Institute, New York
OSTI ID:
5741339
Journal Information:
Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 219:4590; ISSN SCIEA
Country of Publication:
United States
Language:
English

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Related Subjects

560122 -- Radiation Effects on Cells-- Internal Source-- (-1987)
560161* -- Radionuclide Effects
Kinetics
& Toxicology-- Man
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
AGE GROUPS
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
AZINES
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CYCLE
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
ELDERLY PEOPLE
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HUMAN POPULATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MINORITY GROUPS
MITOGENS
MUCOPROTEINS
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
POPULATIONS
PROTEINS
PYRIMIDINES
RADIOBIOLOGY
RADIOSENSITIVITY
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS