Temperature dependence of membrane permeability in cultured cells exposed to benzene and phenol

Journal Article · Fri Oct 15 00:00:00 EDT 1982 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(82)92090-3· OSTI ID:5741003

Walum, E

The effects of benzene (20mM) and phenol (20mM) on the passive membrane permeability of cultured cells of neuronal (N1E115), glial (138MG), muscle (L6) and liver (BRL123) origin were studied. Permeability changes were assayed as alterations in the release of radioactivity from cells preloaded with (/sup 3/H)2-deoxy-D-glucose. Temperature plots of the efflux revealed that benzene and phenol caused altered flux rates in N1E115 cells without affecting the activation energy. Also in 138 MG cells the activation energy was unaffected by both compounds, while phenol decreased the flux rates. Benzene had no effect on the efflux in L6 cultures, whereas a low activation energy efflux was induced by phenol. In BRL123 cells both benzene and phenol induced a dual (high and low) activation energy efflux.

• Research Organization: Univ. of Stockholm, Sundbyberg, Sweden
• OSTI ID: 5741003
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 108:3; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AROMATICS
BENZENE
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
HYDROCARBONS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANES
ORGANIC COMPOUNDS
PERMEABILITY
PHENOL
PHENOLS
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS