Evidence that cultured airway smooth muscle cells contain bradykinin B2 and B3 receptors

Journal Article · Thu Feb 28 23:00:00 EST 1991 · American Journal of Respiratory Cell and Molecular Biology; (USA)

DOI:https://doi.org/10.1165/ajrcmb/4.3.273· OSTI ID:5962445

Farmer, S G; Ensor, J E; Burch, R M ^[1]

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1. Nova Pharmaceutical Corporation, Baltimore, MD (USA)

We examined bradykinin-induced 45Ca2+ efflux and prostaglandin synthesis in guinea pig tracheal smooth muscle cells maintained in tissue culture. We also studied the effects of a B1 receptor agonist and antagonist, a B2 receptor antagonist, and the cyclooxygenase inhibitor indomethacin. In cultured smooth muscle cells, bradykinin (0.1 nM to 10 microM) stimulated efflux of 45Ca2+ and induced the synthesis of prostaglandin E2 and the prostacyclin metabolite 6-keto-prostaglandin F1 alpha. DesArg9-bradykinin, a B1 receptor agonist, had no effect on 45Ca2+ efflux or prostaglandin synthesis, and no responses to bradykinin were unaffected by the B1 receptor antagonist desArg9-(Leu8)-bradykinin. Indomethacin (1 microM) abolished bradykinin-induced prostaglandin synthesis but was without effect on 45Ca2+ efflux. NPC 567 (DArg(Hyp3,DPhe7)-bradykinin), a B2 receptor antagonist, had no effect on bradykinin-induced 45Ca2+ efflux, but abolished prostaglandin synthesis. Unlike in membranes prepared freshly from guinea pig tracheal smooth muscle, the B2 receptor antagonist inhibited completely (Ki, 12 nM) binding of (3H)-bradykinin to membranes prepared from cultured tracheal smooth cells. These data suggest that tracheal smooth muscle cells, maintained in culture, express B2 receptors that mediate bradykinin-induced prostaglandin synthesis. The observation that bradykinin-induced efflux of calcium ions was unaffected by B1 or B2 antagonists provides further evidence that airway smooth muscle may contain a novel B3 receptor.

• OSTI ID: 5962445
• Journal Information: American Journal of Respiratory Cell and Molecular Biology; (USA), Journal Name: American Journal of Respiratory Cell and Molecular Biology; (USA) Vol. 4:3; ISSN 1044-1549; ISSN AJRBE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GUINEA PIGS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
KININS
MAMMALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
MUSCLES
NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROSTAGLANDINS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
RODENTS
SYNTHESIS
TISSUE CULTURES
TRACER TECHNIQUES
TRACHEA
VERTEBRATES