Tritiated 2-deoxy-D-glucose as a probe for cell membrane permeability studies
Tritiated 2-deoxy-D-glucose was taken up and phosphorylated by cultured cells of neuronal (NIE 115), glial (138 MG), muscle (L 6) and liver (BRL 123) origin. Upon perfusion the cells slowly released 2-deoxy-D-glucose 6-phosphate. The following values for rate constants, half-lives, and activation energies for the efflux were obtained: NIE 115: 0.0048 min/sup -1/, 143 min, and 72 kJ mol/sup -1/; 138 MG: 0.0013 min/sup -1/, 547 min, and 85 kJ mol/sup -1/; L 6: 0.0022 min/sup -1/, 311 min, and 60 kJ mol/sup -1/; and BRL 123: 0.0013 min/sup -1/, 528 min and 63 kJ mol/sup -1/. When the cultures were perfused with buffer containing Triton X-100 a time- and concentration-dependent increase in the rate of efflux of 2-deoxy-D-glucose 6-phosphate was obtained. It is suggested that 2-deoxy-D-(/sup 3/H)glucose can be used as a probe in studies of general cell membrane permeability changes.
- Research Organization:
- Univ. of Stockholm, Sweden
- OSTI ID:
- 6727072
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 120:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
DISEASES
GLANDS
GLIOMAS
GLUCOSE
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
MEMBRANES
MICE
MONOSACCHARIDES
MUSCLES
NEOPLASMS
NERVE CELLS
ORGANIC COMPOUNDS
ORGANS
PERMEABILITY
PHOSPHORYLATION
REACTION KINETICS
RODENTS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES