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Effect of radiation on the regulation of sodium-dependent glucose transport in LLC-PK1 epithelial cell line: possible model for gene expression

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576545· OSTI ID:5738747
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Low concentrations of glucose induce cultured kidney epithelial cells (LLC-PK1) to produce hexose transport proteins. We have investigated the effects of ionizing radiation on this induction process in plateau-phase cultures. The induced production of hexose transporters, requiring approximately 6 to 9 days for complete expression, can be inhibited by irradiation during the first 4 days. After the fourth postinduction day, radiation sensitivity decreases with almost no radiation effect on the induction of hexose transport apparent by the sixth day of the induction period. The D0 value associated with the induction block is approximately 25 Gy, a value which is considerably greater than that necessary to inhibit cell replication. Hexose transport, itself resistant to ionizing radiation at doses in excess of 100 Gy, is sensitive to cycloheximide throughout the induction period. The sensitivity to cycloheximide decreases during the last 2 days of the induction period, approximately 1 day after the reduction in radiosensitivity. Based on these properties hexose transport may be a convenient model for the study of radiation effects upon gene expression in this cell line.
Research Organization:
Armed Forces Radiobiology Research Institute, Bethesda, MD
OSTI ID:
5738747
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 2; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOHYDRATES
CELL CULTURES
CYCLOHEXIMIDE
DNA
DOMESTIC ANIMALS
DRUGS
EPITHELIUM
FUNGICIDES
GLUCOSE
HEXOSES
IN VITRO
KIDNEYS
MAMMALS
MEMBRANE TRANSPORT
MONOSACCHARIDES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
PROTEINS
RADIATION EFFECTS
RADIOSENSITIVITY
SACCHARIDES
SWINE
TISSUES
VERTEBRATES