Effect of radiation on the regulation of sodium-dependent glucose transport in LLC-PK/sub 1/ epithelial cell line: possible model for gene expression

Technical Report · Wed Jan 01 04:00:00 EST 1986

OSTI ID:5526202

Moran, A; Davis, L; Hagan, M

Low concentrations of glucose-cultured kidney epithelial cells (LLC-PK/sub 1/) to produce hexose-transport proteins. The effects of ionizing radiation were investigated on this induction process in plateau-phase cultures. The induced production of hexose transporters, requiring approximately 6 to 9 days for complete expression, can be inhibited by irradiation during the first 4 days. After the fourth post-induction day, radiation sensitivity decreases with almost no radiation effect on the induction of hexose transport apparent by the sixth day of the induction period. The D/sub o/ value associated with the induction block is approximately 25 Gy, a value which is considerably greater than necessary to inhibit cell replication. Hexose transport, itself resistant to ionizing radiation at doses in excess of 100 Gy, is sensitive to cycloheximide throughout the induction period. The sensitivity to cycloheximide decreases during the last 2 days of the induction period, approximately 1 day after the reduction in radiosensitivity. Based on these properties hexose transport may be a convenient model for the study of radiation effects upon gene expression in this cell line.

• Research Organization: Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
• OSTI ID: 5526202
• Report Number(s): AD-A-166939/9/XAB
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ALKALI METALS
ANIMAL TISSUES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOHYDRATES
ELEMENTS
EPITHELIUM
GENES
GLUCOSE
HEXOSES
KIDNEYS
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PRODUCTION
PROTEINS
RADIATION EFFECTS
RADIOSENSITIVITY
SACCHARIDES
SODIUM
TISSUES