Role of cell replication in regulation of Na-coupled hexose transport in LLC-PK/sub 1/ epithelial cells
Technical Report
·
OSTI ID:5526213
The glucose concentration in growth medium has been shown to regulate the number of sodium-coupled glucose transporters in LLC-PK/sub 1/ epithelial cells. Epithelia grown in high concentrations of glucose express fewer transporters than epithelia grown in low concentrations of glucose. In this present work, the effect of a dose of ionizing radiation sufficient to block the incorporation of thymidine was examined in order to gauge the importance of cell replication in the hexose-transport regulatory process. The low rate of thymidine incorporation in the plateau phase was completely eliminated by ionizing radiation. Under conditions of irradiation that completely blocked thymidine incorporation, down-regulation, namely the loss of alpha-methylglucoside-concentrating capacity, brought about by switching the epithelium from low to high glucose-containing medium, is independent of the irradiation and therefore most likely is also independent of cell replication. In contrast, the up-regulatory phenomenon is strongly impaired by radiation. This impairment may be due to specific radiation impairment of gene expression necessary for the up-regulatory process. It is apparent from the dose-response data that up-regulation is not inhibited by irradiation in a simple manner and is not inhibited at the same radiation dose as cell replication.
- Research Organization:
- Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
- OSTI ID:
- 5526213
- Report Number(s):
- AD-A-166858/1/XAB
- Country of Publication:
- United States
- Language:
- English
Similar Records
Role of cell replication in regulation of Na-coupled hexose transport in LLC-PK1 epithelial cells
Effect of radiation on the regulation of sodium-dependent glucose transport in LLC-PK/sub 1/ epithelial cell line: possible model for gene expression
Effect of radiation on the regulation of sodium-dependent glucose transport in LLC-PK1 epithelial cell line: possible model for gene expression
Journal Article
·
Fri Jan 31 23:00:00 EST 1986
· Am. J. Physiol.; (United States)
·
OSTI ID:5528865
Effect of radiation on the regulation of sodium-dependent glucose transport in LLC-PK/sub 1/ epithelial cell line: possible model for gene expression
Technical Report
·
Tue Dec 31 23:00:00 EST 1985
·
OSTI ID:5526202
Effect of radiation on the regulation of sodium-dependent glucose transport in LLC-PK1 epithelial cell line: possible model for gene expression
Journal Article
·
Fri Jan 31 23:00:00 EST 1986
· Radiat. Res.; (United States)
·
OSTI ID:5738747
Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ALKALI METALS
ANIMAL TISSUES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOHYDRATES
CELL DIVISION
ELEMENTS
EPITHELIUM
GENES
GLUCOSE
HETEROCYCLIC COMPOUNDS
HEXOSES
IONIZING RADIATIONS
METALS
MONOSACCHARIDES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
SACCHARIDES
SODIUM
THYMIDINE
TISSUES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ALKALI METALS
ANIMAL TISSUES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOHYDRATES
CELL DIVISION
ELEMENTS
EPITHELIUM
GENES
GLUCOSE
HETEROCYCLIC COMPOUNDS
HEXOSES
IONIZING RADIATIONS
METALS
MONOSACCHARIDES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
SACCHARIDES
SODIUM
THYMIDINE
TISSUES