Chicken ovalbumin gene fused to a herpes simplex virus. cap alpha. promoter and linked to a thymidine kinase gene is regulated like a viral gene

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.3.233· OSTI ID:5727066

Post, L E; Norrild, B; Simpson, T; Roizman, B

The authors describe a system for the introduction, selection, and expression of eucaryotic genes in higher eucaryotic cells. The carrier consisted of the herpes simplex virus 1 (HSV-1) tk gene covalently linked to an HSV-1 ..cap alpha.. promoter directed away from the tk gene. In this study they fused to the ..cap alpha.. promoter the 5' transcribed noncoding sequences and the coding sequences of the chicken oviduct ovalbumin gene. Cells converted to the TK/sup +/ phenotype with this chimeric fragment produced an ovalbumin precursor which was processed and secreted into the extracellular fluid. The ovalbumin gene utilized the HSV-1 ..cap alpha.. promoter and was regulated as a viral gene inasmuch as inversion of the genomic DNA relative to the ..cap alpha.. promoter resulted in no ovalbumin synthesis, and production of ovalbumin was enhanced after superinfection with HSV-1. Synthesis of ovalbumin was not detected when cDNA was linked to the HSV-1 ..cap alpha.. promoter. The carrier system described in this study is suitable for introduction, selection, and expression of eucaryotic genes whose natural promoter is either weak or requires the presence of regulatory elements which may be absent from undifferentiated cells in culture.

• Research Organization: The Marjorie B. Kovler Viral Oncology Labs., The Univ. of Chicago, Chicago, IL 60637
• OSTI ID: 5727066
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 2:3; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AZINES
BIOSYNTHESIS
BIOTECHNOLOGY
BIRDS
CARBOHYDRATES
CELL CULTURES
CHICKENS
CLONING
CODONS
DISEASES
DNA SEQUENCING
DNA-CLONING
ENZYMES
FOWL
GENE AMPLIFICATION
GENE OPERONS
GENE REGULATION
GENE REPRESSORS
GENES
GLUCOPROTEINS
HERPES SIMPLEX
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
IMMOBILIZED CELLS
INFECTIOUS DISEASES
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OVALBUMIN
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SKIN DISEASES
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
THYMIDINE
TRANSCRIPTION
TRANSFERASES
VERTEBRATES
VIRAL DISEASES
VIRUSES