Expression of herpes simplex virus. beta. and. gamma. genes integrated in mammalian cells and their induction by an. cap alpha. gene product

Journal Article · Tue Nov 01 04:00:00 EST 1983 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.3.11.2028· OSTI ID:5877796

Sandri-Goldin, R M; Goldin, A L; Holland, L E

The proteins of herpes simplex virus type 1 (HSV-1) form three kinetic groups termed ..cap alpha..,..beta..,and ..gamma.., whose synthesis is regulated in a cascade fashion, ..cap alpha.. products are synthesized first during infection, and they are required for synthesis of ..beta.. and ..gamma.. proteins. To examine the expression of several HSV-1 ..beta.. and ..gamma.. genes in the absence of ..cap alpha.. functions, we transferred into mammalian cells a plasmid containing a region of the HSV-1 genome that codes for only ..beta.. and ..gamma.. genes (0.315 to 0.421 map units). The authors found stable integration of at least one copy of the intact plasmid in each cell line. Four HSV-1 transcripts of the ..beta.. and ..gamma.. classes were transcribed constitutively in the cells, including the genes for glycoprotein B and DNA-binding protein. No constitutive synthesis of these two proteins could be demonstrated, however. The integrated HSV-1 genes responded to viral regulatory signals in that they could be induced by infection with HSV-1 mutants resulting in a high level of synthesis of both glycoprotein B and DNA-binding protein. The HSV-1 ..cap alpha.. gene product ICP4 was necessary for this induction, and it was found to be most efficient at a low multiplicity of infection. Functional expression of four genes was demonstrated in that the cell lines complemented infecting HSV-1 temperature-sensitive mutants. The same genes were not available for homologous recombination with infecting virus, however, since no recombinant wild-type virus could be detected. These data demonstrate that HSV-1 ..beta.. and ..gamma.. genes can be transcribed in the absence of ..cap alpha.. functions in mammalian cells, but that they still respond to HSV-1 regulatory signals such as the ..cap alpha.. gene product ICP4.

• Research Organization: Dept. of Microbiology, Univ. of California, Irvine, CA 92717
• OSTI ID: 5877796
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 3:11; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CARBOHYDRATES
CELL CONSTITUENTS
CELL CULTURES
CLASSIFICATION
DISEASES
DNA
FUNCTIONS
GENE REGULATION
GENES
GLUCOPROTEINS
HERPES SIMPLEX
INFECTIOUS DISEASES
INFECTIVITY
MAMMALS
MICROORGANISMS
MOLECULAR BIOLOGY
MUTANTS
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PLASMIDS
PROTEINS
RECOMBINANT DNA
SACCHARIDES
SKIN DISEASES
SYNTHESIS
TEMPERATURE EFFECTS
VERTEBRATES
VIRAL DISEASES
VIRUSES