New commercial method for the enzymatic determination of creatinine in serum and urine evaluated: Comparison with a kinetic Jaffe method and isotope dilution-mass spectrometry

Journal Article · Mon May 01 04:00:00 EDT 1989 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5723561

Lindbaeck, B B; Bergman, A

We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffe method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID-MS-calibrated serum standard was used. Both the enzymatic and the Jaffe method correlated well with ID-MS, except for sera with high concentrations of bilirubin. Ethyl acetoacetate, acetone, and glucose in serum interfered somewhat with the Jaffe method but not with the enzymatic method. We conclude that the present enzymatic method has merit as compared with a Jaffe method for routine work, but is more expensive.

• Research Organization: Huddinge Univ. Hospital (Sweden)
• OSTI ID: 5723561
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 35:5; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AZOLES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BIOSYNTHESIS
BLOOD SERUM
BODY
BODY FLUIDS
COMPARATIVE EVALUATIONS
CREATININE
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROLASES
IMIDAZOLES
IMINES
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KIDNEYS
MAMMALS
MAN
MASS SPECTROSCOPY
MATERIALS
MEASURING METHODS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PRIMATES
REAGENTS
SPECTROSCOPY
SYNTHESIS
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES