Precision of glucose measurements in control sera by isotope dilution/mass spectrometry: proposed definitive method compared with a reference method

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6025317

Pelletier, O; Arratoon, C

This improved isotope-dilution gas chromatographic/mass spectrometric (GC/MS) method, in which (/sup 13/C)glucose is the internal standard, meets the requirements of a Definitive Method. In a first study with five reconstituted lyophilized sera, a nested analysis of variance of GC/MS values indicated considerable among-vial variation. The CV for 32 measurements per serum ranged from 0.5 to 0.9%. However, concentration and uncertainty values (mmol/L per gram of serum) assigned to one serum by the NBS Definitive Method (7.56 +/- 0.28) were practically identical to those obtained with the proposed method (7.57 +/- 0.20). In the second study, we used twice more (/sup 13/C)glucose diluent to assay four serum pools and two lyophilized sera. The CV ranged from 0.26 to 0.5% for the serum pools and from 0.28 to 0.59% for the lyophilized sera. In comparison, results by the hexokinase/glucose-6-phosphate dehydrogenase reference method agreed within acceptable limits with those by the Definitive Method but tended to be slightly higher (up to 3%) for lyophilized serum samples or slightly lower (up to 2.5%) for serum pools.

• Research Organization: Health Protection Branch, Health and Welfare, Ottawa, Ontario
• OSTI ID: 6025317
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 33:8; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACCURACY
ALDEHYDES
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
ENZYMES
EVEN-ODD NUCLEI
GAS CHROMATOGRAPHY
GLUCOSE
HEXOSES
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
LIGHT NUCLEI
MASS SPECTROSCOPY
MEASURING METHODS
MONOSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
SACCHARIDES
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES