Lysophosphatidylcholines containing polyunsaturated fatty acids were found as Na/sup +/,K/sup +/-ATPase inhibitors in acutely volume-expanded hog
Na/sup +/,K/sup +/-ATPase inhibitors activities against the specific binding of ouabain to Na/sup +/,K/sup +/-ATPase and /sup 86/Rb uptake into hog erythrocytes have been purified from the plasma of acutely saline-infused hog. The purifications were performed by a combination of Amberlite XAD-2 adsorption chromatography and four steps of high-performance liquid chromatography with four different types of columns. Fast atom bombardment (FAB) mass and proton NMR spectrometric studies identified the purified substances as ..gamma..-arachidoyl- (LPCA(..gamma..), 34%), ..beta..-arachidoyl- (LPCA(..beta..), 4%), ..gamma..-linoleoyl- (LPCL, 33%), and ..gamma..-oleoyl- (LPCO, 25%) lysophosphatidylcholine, expressed in molar ratio in the plasma. Small amounts of ..gamma..-docosapentaenoyl-, ..gamma..-eicosatrienoyl-, and ..gamma..palmitoyllysophosphatidylcholine were also detected by both FAB mass and /sup 1/H NMR spectrometric studies. The inhibition of Na/sup +/,K/sup +/-ATPase activity due to these compounds was always more sensitive than that of both ouabain-binding and /sup 86/Rb uptake activities. The ouabain-displacing activity in plasma due to these compounds increased with time during saline infusion. The maximal plasma level was approximately 10 times higher than that in the preinfusion plasma sample. Although these results suggest that ..gamma..-acyl-LPC's with long-chain polyunsaturated fatty acids are not simple competitive inhibitors to Na/sup +/,K/sup +/-ATPase, these compounds could be implicated in the pathogenesis of the circulation abnormality through the modulation of membrane enzyme.
- Research Organization:
- Vanderbilt Univ., Nashville, TN
- OSTI ID:
- 5723109
- Journal Information:
- Biochemistry; (United States), Vol. 26:10
- Country of Publication:
- United States
- Language:
- English
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ENZYME INHIBITORS
MOLECULAR STRUCTURE
PURIFICATION
RUBIDIUM 86
UPTAKE
ATP
ATP-ASE
CHOLINE
ERYTHROCYTES
LIQUID COLUMN CHROMATOGRAPHY
NUCLEAR MAGNETIC RESONANCE
ORGANIC PHOSPHORUS COMPOUNDS
OUABAIN
PHOSPHORUS 32
PROTONS
RUBIDIUM CHLORIDES
ACID ANHYDRASES
ALCOHOLS
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
AMINES
AMMONIUM COMPOUNDS
BARYONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
GLYCOSIDES
HADRONS
HALIDES
HALOGEN COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAGNETIC RESONANCE
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEONS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHORUS ISOTOPES
QUATERNARY COMPOUNDS
RADIOISOTOPES
RESONANCE
RUBIDIUM COMPOUNDS
RUBIDIUM ISOTOPES
SEPARATION PROCESSES
STROPHANTHINS
550201* - Biochemistry- Tracer Techniques