Unusual estrogen-binding protein of the rat liver: effects on nuclear accumulation of estradiol-receptor complexes in vitro

Journal Article · Mon Mar 10 04:00:00 EST 1986 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5722621

Smirnov, A N; Shchelkunova, T A; Rozen, V B

The accumulation of (/sup 3/H)estradiol-receptor complexes by liver nuclei after preliminary incubation of the hormone with rat liver cytosol was investigated. It was shown that the addition of a purified preparation of the unusual estrogen-binding protein (UEBP) isolated from the liver of males to the cytosol of females induces a dose-dependent inhibition of the subsequent accumulation of specifically bound (/sup 3/H)estradiol in the nuclei. The addition of 1.5 ..mu..M 2..cap alpha..-hydroxytestosterone, testosterone, 1-dehydrotestosterone, 5..cap alpha..-androstane-3..cap alpha..,17..beta..-diol, and 5..cap alpha..-dihydrotestosterone - a compound possessing pronounced affinity for the UEBP - to the liver cytosol of males leads to a 2-5-fold increase in subsequent nuclear accumulation of the estrogen-receptor complexes. Androsterone, which does not interact with the UEBP, does not exhibit such an effect. When liver cytosol of females, lacking the UEBP, is used, the indicated steroids are ineffective with respect to estrogen reception. It was concluded that the UEBP of the liver of male rats is capable of modulating estrogen reception.

• Research Organization: M.V. Lomonosov Moscow State Univ., USSR
• OSTI ID: 5722621
• Journal Information: Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:9; ISSN BIORA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL ACCUMULATION
BODY
CELL CONSTITUENTS
CELL NUCLEI
CHEMISTRY
COMPLEXES
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ESTRADIOL
ESTRANES
ESTROGENS
GLANDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LABELLING
LIVER
LIVER CELLS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIORECEPTOR ASSAY
RATS
RECEPTORS
RESPONSE MODIFYING FACTORS
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
TESTOSTERONE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES