In vitro metabolism of androstenedione and identification of endogenous steroids in Helix aspersa

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Gen. Comp. Endocrinol.; (United States)

DOI:https://doi.org/10.1016/0016-6480(87)90253-X· OSTI ID:6178828

Le Guellec, D; Thiard, M C; Remy-Martin, J P; Deray, A; Gomot, L; Adessi, G L

In vitro metabolism of androstenedione in gonads of juvenile and adult Helix aspersa has been investigated. The conversion of (/sup 3/H)androstenedione into testosterone, 5 alpha-dihydrotestosterone, androsterone, and estriol was demonstrated. In juvenile animals testosterone (59.8%) is the major metabolite whereas in adult animals androsterone (18.8%) is. The following endogenous steroids have been identified by gas chromatography-mass spectrometry in adult gonads: androsterone, dehydroepiandrosterone, androstenedione, 3 alpha-androstanediol, estrone, estradiol-17 beta, and estriol. The levels of testosterone, 5 alpha-dihydrotestosterone, androstenedione, and progesterone have been measured by RIAs in gonads and hemolymph. Their levels vary with the physiological stage: the gonadal and circulating levels of testosterone decrease with the sexual maturation whereas the 5 alpha-dihydrotestosterone increases. These differences observed in metabolism and in level of steroids between the juvenile and the adult snails allow us to suppose that these steroids have a biological role.

• Research Organization: Inserm U 198, Besancon, France
• OSTI ID: 6178828
• Journal Information: Gen. Comp. Endocrinol.; (United States), Journal Name: Gen. Comp. Endocrinol.; (United States) Vol. 66:3; ISSN GCENA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
ANDROGENS
ANDROSTANES
ANDROSTENEDIONE
ANDROSTERONE
ANIMALS
AQUATIC ORGANISMS
BIOSYNTHESIS
CHROMATOGRAPHY
ESTRANES
ESTRIOL
ESTROGENS
GAS CHROMATOGRAPHY
GONADS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
IN VITRO
INVERTEBRATES
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MASS SPECTROSCOPY
METABOLISM
METABOLITES
MOLLUSCS
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SEPARATION PROCESSES
SNAILS
SPECTROSCOPY
STEROID HORMONES
STEROIDS
SYNTHESIS
TESTOSTERONE
TRACER TECHNIQUES
TRITIUM COMPOUNDS