Estrogen binding protein activity in Morris hepatoma 7777 compared with normal rat liver

Journal Article · Fri Jun 01 04:00:00 EDT 1984 · Gastroenterology; (United States)

OSTI ID:6817086

Francavilla, A; Eagon, P K; DiLeo, A; Van Thiel, D H; Ove, P; Wu, S K; Sax, S A; Starzl, T E

Estrogen binding protein activities were determined in the cytosol from adult male Buffalo rat liver and Morris hepatoma 7777. Estrogen receptors were prepared using the protamine sulfate precipitation technique of Chamness. The ability of various unlabeled steroids competing with (/sup 3/H)estradiol was examined to establish the binding specificity. Estradiol binding in Morris hepatoma 7777 cytosol was greatly decreased compared with that present in hepatic cytosol prepared from normal rat liver. The receptor concentration expressed as femtomoles per milligram of cytoplasmic protein was 31.1 +/- 2.9 SD for normal rat liver and 0.41 +/- 0.88 SD for the hepatoma. Gel filtration chromatography revealed the presence of an estrogen binder in hepatoma cytosol which was not present in either normal liver or in the protamine sulfate precipitates of hepatoma cytosol. The molecular weight, binding specificity, and precipitation of this protein by specific antiserum suggests that it is alpha-fetoprotein.

• Research Organization: Department of Gastroenterology, University of Bari, Italy
• OSTI ID: 6817086
• Journal Information: Gastroenterology; (United States), Journal Name: Gastroenterology; (United States) Vol. 86:6; ISSN GASTA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISEASES
ESTROGENS
GLANDS
HEPATOMAS
HORMONES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAMMALS
NEOPLASMS
ORGANS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SEPARATION PROCESSES
STEROID HORMONES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES