Cellular regulation of ADP-ribosylation of proteins: 3. Selective augmentation of in vitro ADP-ribosylation of histone H3 in murine thymic cells after in vivo emetine treatment

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90362-5· OSTI ID:5714575

Sooki-Toth, A; Banfalvi, G; Staub, M; Antoni, F ^[1]; Szoelloesi, J ^[2]; Kirsten, E ^[3]; Kun, E ^[4]

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1. Semmelweis Univ., Budapest (Hungary)
2. Univ. Medical School of Debrecen (Hungary)
3. State Univ. of San Francisco, Tiburon, CA (United States)
4. Univ. of California, San Francisco (United States)

Thymic cells were isolated at intervals of between 0 and 144 h from mice that received one intraperitoneal injection of emetine, and thymus weight, incorporation of ({sup 14}C)leucine into proteins and ({sup 3}H)thymidine into DNA in intact thymic cells, as well as initial rates of protein ADP-ribosylation in permeabilized cells were simultaneously monitored. The effect of emetine as an inhibitor of protein synthesis corresponds to the induction of sequential cellular events, such as cell exit and remigration, by other antimitotic agents and produces an activation of proliferation of cells reentering into this organ. Proliferation, as demonstrated by a large increase in DNA synthesis and entrance into S phase, was kinetically related to an apparent increase in poly (ADP-ribose) polymerase activity in thymic cells and a highly significant in vitro ADP-ribosylation of histone H3. since no DNA fragmentation occurred in thymic cells, as tested by a fluorometric technique it is probable that a selective activation of poly (ADP-ribose) polymerase may have been induced in cells that undergo differentiation and proliferation while repopulating thymus.

• OSTI ID: 5714575
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 184:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
ALDEHYDES
AMINO ACIDS
ANIMAL CELLS
AZINES
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL ACTIVATION
DNA
DNA POLYMERASES
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HETEROCYCLIC COMPOUNDS
HISTONES
LABELLED COMPOUNDS
LEUCINE
MONOSACCHARIDES
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENTOSES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
PYRIMIDINES
RIBOSE
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
THYMIDINE
THYMUS CELLS
TRANSFERASES
UPTAKE