Further evidence for poly-ADP-ribosylated histones as DNA suppressors

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5207925

Yu, F L; Geronimo, I H; Bender, W; Meginniss, K E

For many years histones have been considered to be the gene suppressors in eukaryotic cells. Recently, the authors have found strong evidence indicating that poly-ADP-ribosylated histones, rather than histones, are the potent inhibitors of DNA-dependent RNA synthesis. They now report additional evidence for this concept: 1) using histone inhibitor isolated directly from nuclei, the authors are able to confirm their earlier findings that the inhibitor substances are sensitive to pronase, snake venom phosphodiesterase digestion and 0.1N KOH hydrolysis, and are resistant to DNase I and RNase A digestion, 2) the O.D. 260/O.D.280 ratio of the histone inhibitor is between pure protein and nuclei acid, suggesting the inhibitor substance is a nucleoprotein hybrid. This result directly supports the fact that the isolated histone inhibitor is radioactive poly (ADP-ribose) labeled, 3) commercial histones show big differences in inhibitor activity. The authors believe this reflects the variation in poly-ADP-ribosylation among commercial histones, and 4) 0.1N KOH hydrolysis eliminates the poly (ADP-ribose) radioactivity from the acceptor proteins as well as histone inhibitor activity. Yet, on gel, the inhibitor shows identical histone bands and stain intensity before and after hydrolysis, indicating the histones per se are qualitatively and quantitatively unaffected by alkaline treatment. This result strongly suggests that histones themselves are not capable of inhibiting DNA-dependent RNA synthesis.

• Research Organization: Univ. of Illinois College of Medicine, Rockford
• OSTI ID: 5207925
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
ALDEHYDES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DNA
HISTONES
HYDROLYSIS
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LYSIS
MONOSACCHARIDES
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOTIDES
ORGANIC COMPOUNDS
PENTOSES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RIBOSE
RNA
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
TRACER TECHNIQUES