Poly(ADP-ribose) and the response of cells to ionizing radiation

Conference · Tue Jan 01 04:00:00 EST 1985 · Radiat. Res.; (United States)

OSTI ID:5930562

Oleinick, N L; Evans, H H

The activity of poly(ADP-ribose) polymerase is stimulated by DNA damage resulting from treatment of cells with ionizing radiation, as well as with DNA-damaging chemicals. The elevated polymerase activity can be observed at doses lower than those necessary for measurable reduction in cellular NAD concentration. Several nuclear proteins, including the polymerase itself, are poly(ADP-ribosylated) at elevated levels in irradiated Chinese hamster cells. The addition of inhibitors of poly(ADP-ribose) polymerase to irradiated cells has been found to sensitize the cells to the lethal effects of the radiation, to inhibit the repair of potentially lethal damage, and to delay DNA strand break rejoining. Because of the nonspecificity of the inhibitors, however, it is as yet unknown whether their effects are directly related to the inhibition of poly(ADP-ribose) polymerase, to interference with the poly(ADP-ribosylation) of one or more chromosomal proteins, or to effects unrelated to the poly(ADP-ribosylation) process. The data are consistent with the involvement of poly(ADP-ribose) in the repair of radiation damage, but the nature of this involvement remains to be elucidated.

• Research Organization: Case Western Reserve Univ. School of Medicine, Cleveland, OH
• DOE Contract Number: AC02-77EV04472
• OSTI ID: 5930562
• Report Number(s): CONF-8403123-
• Conference Information: Journal Name: Radiat. Res.; (United States) Journal Volume: 101:1
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL CELLS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CHROMOSOMAL ABERRATIONS
COENZYMES
DAYS LIVING RADIOISOTOPES
DNA
DNA REPAIR
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ENZYMES
GAMMA RADIATION
IONIZING RADIATIONS
ISOTOPES
LIGHT NUCLEI
MONOSACCHARIDES
MUTATIONS
NAD
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PENTOSES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
REVIEWS
RIBOSE
SACCHARIDES
SISTER CHROMATID EXCHANGES
STRAND BREAKS
TRANSFERASES
TUMOR CELLS