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Title: Dual-isotope method for determination of human zinc absorption: the use of a test meal of turkey meat

Abstract

The percentage of /sup 65/Zn taken up (absorbed) from extrinsically labeled turkey meat was calculated from the amounts of /sup 65/Zn and a nonabsorbed /sup 51/Cr marker present in the body or in a single stool specimen after 1-2 d. /sup 51/CrCl/sub 3/ proved to be a suitable marker for unabsorbed /sup 65/Zn and so the early determination of /sup 65/Zn absorption was possible. With stool counting, /sup 65/Zn absorption data from first stool samples after 1-2 d were accurate as judged by correlation with the amount of /sup 65/Zn in the body 7-10 d later (retention); results from subsequent stools gave lower absorption values due to the early excretion of some absorbed /sup 65/Zn. The dual-isotope method gave reproducible results when four successive tests of zinc absorption were carried out in a group of six subjects. The average (mean +/- SD) /sup 65/Zn absorption from turkey meals containing 31 mumol (2 mg) and 46 mumol (3 mg) of zinc was 39 +/- 8% and 29 +/- 6%, respectively, measured by stool counting; /sup 65/Zn absorption and retention correlated well in both studies. A series of different beverages was given in place of water with the turkey meal. Orange juicemore » significantly reduced /sup 65/Zn absorption and milk also showed this tendency, but tea, whiskey, wine or beer had no significant effect on the absorption of /sup 65/Zn from the turkey meal. In groups of subjects the mean ratio of /sup 65/Zn absorption from extrinsically labeled turkey meat on two occasions (1.06) was not significantly different from that of the absorption of extrinsic to intrinsic /sup 65/Zn labels (1.16). The dual-isotope technique with either stool or body counting is suitable for the rapid determination of /sup 65/Zn absorption from extrinsically labeled turkey within 2 d.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. Hospital, London, Ontario
OSTI Identifier:
5709786
Resource Type:
Journal Article
Journal Name:
J. Nutr.; (United States)
Additional Journal Information:
Journal Volume: 115:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MEAT; LABELLING; ZINC; INTESTINAL ABSORPTION; CHROMIUM 51; DUAL-ISOTOPE SUBTRACTION TECHNIQUE; MAN; NUTRITION; RESPONSE MODIFYING FACTORS; TRACER TECHNIQUES; ZINC 65; ABSORPTION; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; CHROMIUM ISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ELEMENTS; EVEN-ODD NUCLEI; FOOD; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; METALS; NUCLEI; PRIMATES; RADIOISOTOPES; UPTAKE; VERTEBRATES; ZINC ISOTOPES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Flanagan, P R, Cluett, J, Chamberlain, M J, and Valberg, L S. Dual-isotope method for determination of human zinc absorption: the use of a test meal of turkey meat. United States: N. p., 1985. Web.
Flanagan, P R, Cluett, J, Chamberlain, M J, & Valberg, L S. Dual-isotope method for determination of human zinc absorption: the use of a test meal of turkey meat. United States.
Flanagan, P R, Cluett, J, Chamberlain, M J, and Valberg, L S. Tue . "Dual-isotope method for determination of human zinc absorption: the use of a test meal of turkey meat". United States.
@article{osti_5709786,
title = {Dual-isotope method for determination of human zinc absorption: the use of a test meal of turkey meat},
author = {Flanagan, P R and Cluett, J and Chamberlain, M J and Valberg, L S},
abstractNote = {The percentage of /sup 65/Zn taken up (absorbed) from extrinsically labeled turkey meat was calculated from the amounts of /sup 65/Zn and a nonabsorbed /sup 51/Cr marker present in the body or in a single stool specimen after 1-2 d. /sup 51/CrCl/sub 3/ proved to be a suitable marker for unabsorbed /sup 65/Zn and so the early determination of /sup 65/Zn absorption was possible. With stool counting, /sup 65/Zn absorption data from first stool samples after 1-2 d were accurate as judged by correlation with the amount of /sup 65/Zn in the body 7-10 d later (retention); results from subsequent stools gave lower absorption values due to the early excretion of some absorbed /sup 65/Zn. The dual-isotope method gave reproducible results when four successive tests of zinc absorption were carried out in a group of six subjects. The average (mean +/- SD) /sup 65/Zn absorption from turkey meals containing 31 mumol (2 mg) and 46 mumol (3 mg) of zinc was 39 +/- 8% and 29 +/- 6%, respectively, measured by stool counting; /sup 65/Zn absorption and retention correlated well in both studies. A series of different beverages was given in place of water with the turkey meal. Orange juice significantly reduced /sup 65/Zn absorption and milk also showed this tendency, but tea, whiskey, wine or beer had no significant effect on the absorption of /sup 65/Zn from the turkey meal. In groups of subjects the mean ratio of /sup 65/Zn absorption from extrinsically labeled turkey meat on two occasions (1.06) was not significantly different from that of the absorption of extrinsic to intrinsic /sup 65/Zn labels (1.16). The dual-isotope technique with either stool or body counting is suitable for the rapid determination of /sup 65/Zn absorption from extrinsically labeled turkey within 2 d.},
doi = {},
journal = {J. Nutr.; (United States)},
number = ,
volume = 115:1,
place = {United States},
year = {1985},
month = {1}
}