Dual-isotope method for determination of human zinc absorption: the use of a test meal of turkey meat

Journal Article · Tue Jan 01 04:00:00 EST 1985 · J. Nutr.; (United States)

OSTI ID:5709786

Flanagan, P R; Cluett, J; Chamberlain, M J; Valberg, L S

The percentage of /sup 65/Zn taken up (absorbed) from extrinsically labeled turkey meat was calculated from the amounts of /sup 65/Zn and a nonabsorbed /sup 51/Cr marker present in the body or in a single stool specimen after 1-2 d. /sup 51/CrCl/sub 3/ proved to be a suitable marker for unabsorbed /sup 65/Zn and so the early determination of /sup 65/Zn absorption was possible. With stool counting, /sup 65/Zn absorption data from first stool samples after 1-2 d were accurate as judged by correlation with the amount of /sup 65/Zn in the body 7-10 d later (retention); results from subsequent stools gave lower absorption values due to the early excretion of some absorbed /sup 65/Zn. The dual-isotope method gave reproducible results when four successive tests of zinc absorption were carried out in a group of six subjects. The average (mean +/- SD) /sup 65/Zn absorption from turkey meals containing 31 mumol (2 mg) and 46 mumol (3 mg) of zinc was 39 +/- 8% and 29 +/- 6%, respectively, measured by stool counting; /sup 65/Zn absorption and retention correlated well in both studies. A series of different beverages was given in place of water with the turkey meal. Orange juice significantly reduced /sup 65/Zn absorption and milk also showed this tendency, but tea, whiskey, wine or beer had no significant effect on the absorption of /sup 65/Zn from the turkey meal. In groups of subjects the mean ratio of /sup 65/Zn absorption from extrinsically labeled turkey meat on two occasions (1.06) was not significantly different from that of the absorption of extrinsic to intrinsic /sup 65/Zn labels (1.16). The dual-isotope technique with either stool or body counting is suitable for the rapid determination of /sup 65/Zn absorption from extrinsically labeled turkey within 2 d.

• Research Organization: Univ. Hospital, London, Ontario
• OSTI ID: 5709786
• Journal Information: J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 115:1; ISSN JONUA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
CHROMIUM 51
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FOOD
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
MAMMALS
MAN
MEAT
METALS
NUCLEI
NUTRITION
PRIMATES
RADIOISOTOPES
RESPONSE MODIFYING FACTORS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES
ZINC
ZINC 65
ZINC ISOTOPES