Excision repair and patch size in UV-irradiated bacteriophage T4

Journal Article · Sun Nov 01 04:00:00 EST 1981 · J. Virol.; (United States)

OSTI ID:5707427

Yarosh, D B ^[1]; Rosenstein, B S; Setlow, R B

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1. National Cancer Institute, Bethesda, MD

We determined the average size of excision repair patches in repair of UV lesions in bacteriophage T4 by measuring the photolysis of bromodeoxyuridine incorporated during repair. The average patch was small, approximately four nucleotides long. In control, experiments with the denV/sub 1/ excision-deificient mutant, we encountered an artifact, a protein(s) which remained bound to phenol-extracted DNA and prevented nicking by the UV-specific endonucleases of Micrococcus luteus and bacteriophage T4.

• OSTI ID: 5707427
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 40:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

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