Measurement of repair patch size by quantitation of nucleotides excised during DNA repair in vivo

Journal Article · Mon Aug 01 04:00:00 EDT 1983 · J. Virol.; (United States)

OSTI ID:5347244

Radany, E H; Friedberg, E C

Escherichia coli uvr/sup -/ cells, prelabeled in their DNA, were infected with phage T4 denV/sup +/ or T4 denV/sup -/ under conditions that preclude phage-mediated degradation of the bacterial chromosome. Measurement of the distribution of acid-soluble radioactivity between pyrimidine dimers and nondimer nucleotides in cell extracts yielded calculated estimates of the average size of excision repair tracts that are in good agreement with the size of repair patches determined by others using direct measurement of repair synthesis.

• Research Organization: Stanford Univ., CA
• DOE Contract Number: AT03-76EV70032
• OSTI ID: 5347244
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 47:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AZINES
BACTERIA
BACTERIOPHAGES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
ESCHERICHIA COLI
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RECOVERY
REPAIR
TRACER TECHNIQUES
VIRUSES