Excision repair and patch size in UV-irradiated bacteriophage T4

Journal Article · Sun Nov 01 04:00:00 EST 1981 · J. Virol.; (United States)

OSTI ID:5363375

Yarosh, D B; Rosenstein, B S; Setlow, R B

We determined the average size of excision repair patches in repair of UV lesions in bacteriophage T4 by measuring the photolysis of bromodeoxyuridine incorporated during repair. The average patch was small, approximately four nucleotides long. In control experiments with the denV1 excision-deficient mutant, we encountered an artifact, a protein(s) which remained bound to phenol-extracted DNA and prevented nicking by the UV-specific endonucleases of Micrococcus luteus and bacteriophage T4.

• Research Organization: Nucleic Acids Section, Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda
• OSTI ID: 5363375
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 40:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIMETABOLITES
AZINES
BACTERIOPHAGES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DNA
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
ULTRAVIOLET RADIATION
URACILS
VIRUSES