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Adrenocorticotropin receptors: Functional expression from rat adrenal mRNA in Xenopus laevis oocytes

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America; (United States)
;  [1]
  1. National Inst. of Health, Bethesda, MD (United States)
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The adrenocorticotropin (ACTH) receptor, which binds corticotropin and stimulates adenylate cyclase and steroidogenesis in adrenocortical cells, was expressed in Xenopus laevis oocytes microinjected with rat adrenal poly(A){sup +} RNA. Expression of the ACTH receptor in individual stage 5 and 6 oocytes was monitored by radioimmunoassay of ligand-stimulated cAMP production. Injection of 5-40 ng of adrenal mRNA caused dose-dependent increases in ACTH-responsive cAMP production. Size fractionation of rat adrenal poly(A){sup +}RNA by sucrose density-gradient centrifugation revealed that mRNA encoding the ACTH receptor was present in the 1.1-to 2.0-kilobase fraction. These data indicate that ACTH receptors can be expressed from adrenal mRNA in Xenopus oocytes and are fully functional in terms of ligand specificity and signal generation. The extracellular cAMP response to ACTH is a sensitive and convenient index of receptor expression. This system should permit more complete characterization and expression cloning of the ACTH receptor.

OSTI ID:
5701147
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:19; ISSN 0027-8424; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550600* -- Medicine
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
AMP
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CENTRIFUGATION
CORTICOSTEROIDS
CYCLASES
DIAGNOSTIC TECHNIQUES
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
GERM CELLS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KETONES
KINETICS
LYASES
MEMBRANE PROTEINS
MESSENGER-RNA
NUCLEIC ACIDS
NUCLEOTIDES
OOCYTES
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
REACTION KINETICS
RECEPTORS
RNA
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES