Exogenous valine reduces conversion of leucine to 3-methyl-1-butanol in Saccharomyces cerevisiae

Journal Article · Tue Feb 01 04:00:00 EST 1983 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5695361

Bigelis, R ^[1]; Weir, P D; Jones, R R.M.; Umbarger, H E

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1. Wake Forest Univ., Winston-Salem, NC

Mutant strains of the yeast Saccharomyces cerevisiae that require branched-chain amino acids must be supplemented with large concentrations (up to 10 mM) of these amino acids to satisfy their nutritional requirements. The utilization of one branched-chain amino acid, leucine, was examined in several leul strains of yeast grown aerobically in a glucose-ammonium salts minimal medium containing a limiting concentration (0.2 mM) of leucine. In this medium, the leucine requirement of the auxotrophic strains could be reduced by valine, another branched-chain amino acid. Increasing the valine concentration increased the cell yields of cultures and also reduced the levels of 3-methyl-1-butanol detected in the medium by gas chromatography. The concentration of 3-methyl-1-butanol was reduced from 122.0 to 48.9 ..mu..M when 5.0 mM valine was supplemented to limiting-leucine cultures. The amino acids isoleucine, threonine, norleucine, norvaline, ..cap alpha..-amino-butyrate, alanine, and glycine also spared the leucine requirement of leucine auxotrophs, most likely because they resemble leucine and competed for its uptake. We propose that leucine analogs restrict the entry and degradation of leucine and thus reduce its conversion to 3-methyl-1-butanol, a major component of fuel oil.

• OSTI ID: 5695361
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 45:2; ISSN AEMID
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINO ACIDS
BIOCHEMISTRY
BIOCONVERSION
BIOSYNTHESIS
BUTANOLS
CARBOXYLIC ACIDS
CHEMISTRY
CHROMATOGRAPHY
FUEL OILS
FUELS
FUNGI
GAS CHROMATOGRAPHY
HYDROXY COMPOUNDS
LEUCINE
LIQUID FUELS
MICROORGANISMS
OILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PETROLEUM PRODUCTS
PLANTS
RESPONSE MODIFYING FACTORS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SEPARATION PROCESSES
SYNTHESIS
VALINE
YEASTS