Inhibition of mouse peritoneal macrophage DNA synthesis by infection with the Arenavirus Pichinde. Interim report
Macrophage DNA synthesis and proliferation occur during the development of cell-mediated immunity and in the early non-specific reaction to infection. Arenaviruses have a predilection for infection of cells of the reticuloendothelial system and in this study we have examined the effect of the arenavirus Pichinde on macrophage DNA synthesis. We have found that infection of mouse peritoneal macrophages with Pichinde caused a profound dose dependent inhibition of the DNA synthesis induced by macrophage growth factor/colony stimulating factor. At a multiplicity of inoculum of five there is a 75-95% inhibition of DNA synthesis. Viable virus is necessary for inhibition since Pichinde inactivated by heat or cobalt irradiation had no effect. Similarly, virus pre-treated with an antiserum to Pichinde was without inhibitory effect. Inhibition was demonstrated by measuring DNA synthesis spectrofluorometrically as well as by 3H-thymidine incorporation. The inhibition of DNA synthesis was not associated with any cytopathology. There was no evidence that the inhibition was due to soluble factors, such as prostaglandins or interferon, released by infected cells. These studies demonstrate, for the first time in vitro, a significant alteration in macrophage function caused by infection with an arenavirus. It is possible that inhibition of macrophage proliferation represents a mechanism by which some microorganisms interfere with host resistance.
- Research Organization:
- Army Medical Research Inst. of Infectious Diseases, Frederick, MD (USA)
- OSTI ID:
- 5687809
- Report Number(s):
- AD-A-126176/7
- Country of Publication:
- United States
- Language:
- English
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DNA
BIOSYNTHESIS
IMMUNITY
BIOLOGICAL MODELS
IN VITRO
INFECTIVITY
INHIBITION
MICE
RETICULOENDOTHELIAL SYSTEM
TRITIUM COMPOUNDS
VIRUSES
ANIMAL TISSUES
ANIMALS
BODY
LABELLED COMPOUNDS
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RODENTS
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques